| Resumen: | Objetivo: Determinar el efecto protector del extracto acuoso de las hojas de Peumus boldus "Boldo" en la toxicidad hepática inducida por rifampicina en ratas Holtzman hembra. Materiales y métodos: Se realizó un estudio experimental completo. Se adquirieron 24 ratas Holtzman hembra, y fueron divididas, aleatoriamente, en 4 grupos: A (control), B (rifampicina 100 mg/kg), C (silimarina 200 mg/kg y rifampicina 100 mg/kg) y D (boldo 160 mg/kg y rifampicina 100 mg/kg). Dicho protocolo se realizó por 14 días. Se analizaron variables clínica, bioquímicas (se realizó perfil hepático antes y después del experimento) y morfológica. Se compararon las medias de los grupos mediante la prueba de Kruskall Wallis y se consideró significativo p<0,05. Resultados: Al finalizar el experimento, las ratas del grupo B perdieron más peso (16,41+/-17,94 gr), tuvieron mayor elevación de bilirrubinas totales (0,25+/-0,13), bilirrubina directa (0,21+/-0,12), TGP (48,83+/-17,72) y sus hígados fueron más congestivos, pesaron y midieron más. Las proteínas totales (0,66+/-0,38) y albúmina (0,33+/-0,35) del grupo B fueron menores comparado con los grupos C y D. No se halló diferencia significativa en cuanto a la bilirrubina indirecta, globulina y TGP. Los hígados del grupo B mostraron mayores cambios histopatológicos (5,33+/-0,51) en comparación con los demás grupos. Conclusiones: el extracto acuoso de las hojas de Peumus boldus posee efecto protector en la toxicidad hepática inducida por rifampicina en ratas Holtzman hembra. (AU)^iesObjective: To determinate the protective effect the aqueous extract of the leaves of Peumus boldus "Boldo" on rifampicin-induced liver toxicity in female Holtzman rats. Materials and methods: A complete experimental study was conducted 24 female Holtzman rats were used. They were divided in 4 groups: A (control), B (rifampicin 100 mg/kg), grupo C (silimarina 200 mg/kg and rifampicin 100 mg/kg) y D (boldo 160 mg/kg and rifampicin 100 mg/kg). Rifampicin was administrated one hour after treatment in groups C and D. This protocol was carried out for 14 days. Clinical, biochemical (liver function test were performed before and after the experiment) and morphological variables were analyzed. Means were compared using the Kruskall Wallis test and results were considered significant when p<0.05. Results: At the end of the experiment, rats from group B were the ones who lost more weight, (16.41+/-17.94 g), showed grater elevation of total bilirubin (0.25+/-0.13), direct bilirubin (0.21+/-0.12), TGP (48.83+/-17.72), and whose livers were found to be more congestive, were bigger and weighed more. Whereas leaves of total proteins (0.66+/-0.38) and albumin (0.33+/-0.35) in group B were lower than in other groups. No significant difference was found in levels of indirect bilirubin, globulin and TGO. More intense morphological changes were found in livers of the group B (5.33+/-0.51) compared with other groups. Conclusions: After analyzing clinical, biochemical and morphological parameter, it can be concluded that the aqueous extract of leaves of Peumus boldus has protective effect on rifampicin-induced liver toxicity in female Holtzman rats. (AU)^ien.
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| Localización: | PE13.1; MB, W, 20.55.A5, O44, ej.1. 010000097980; PE13.1; MB, W, 20.55.A5, O44, ej.2. 010000097981 |
